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Jarrett, Harry
One or more keywords matched the following properties of Jarrett, Harry
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overview Signal transduction, and more specifically the role of Ca2+ and its receptor protein, calmodulin, in cellular regulation We have shown that dystrophin, the protein product of the Duchenne muscular dystrophy gene locus, is a specific calmodulin-binding protein and discovered a signal transduction pathway, which links laminin-a-dystroglycan-ß-dystroglycan-dystrophin-syntrophin-Grb2-Sos1-Rac1-PAK1-MKK4/7-JNK, causing c-jun phosphorylation and activation. This work is supported by National Institutes of Health grant 1R01AR051440. Transcription factor purification and characterization We first coupled DNA to HPLC silica and have made a significant contribution to the purification and characterization of transcription factors. Most recently we developed systematic oligonucleotide trapping, a method for purifying transcription factors to near homogeneity in a single step. Current experiments involve promoter trapping, a method which interfaces DNA-HPLC with proteomic techniques (MALDI-TOF and ESE-ion trap) to characterize the transcription complex of the c-jun promoter. This work is supported by National Institutes of Health grant 1R01GM43609.
One or more keywords matched the following items that are connected to Jarrett, Harry
Item TypeName
Academic Article Methods for proteomic analysis of transcription factors.
Academic Article Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT).
Academic Article Transcription factor proteomics: identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography-electrospray-mass spectrometry analysis.
Academic Article LG4-5 domains of laminin-211 binds alpha-dystroglycan to allow myotube attachment and prevent anoikis.
Academic Article Pleckstrin homology domain 1 of mouse alpha 1-syntrophin binds phosphatidylinositol 4,5-bisphosphate.
Academic Article Repeated probing of Southwestern blots using alkaline phosphatase stripping.
Academic Article Calmodulin-binding proteins also have a calmodulin-like binding site within their structure. The flip-flop model.
Academic Article Temperature dependence of DNA affinity chromatography of transcription factors.
Academic Article Laminin-a1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP-1 and NF-?B transcription factors but also has adverse effects.
Academic Article Trifluoperazine binding to porcine brain calmodulin and skeletal muscle troponin C.
Academic Article T(3): targeted proteomics of DNA-binding proteins.
Academic Article A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene in brown adipocytes.
Academic Article Coupling of deoxyribonucleic acid to solid supports using 3' terminal ribose incorporation.
Academic Article The effects of deletions in the central helix of calmodulin on enzyme activation and peptide binding.
Academic Article Method for trapping affinity chromatography of transcription factors using aldehyde-hydrazide coupling to agarose.
Academic Article Southwestern Blotting Assay.
Academic Article Interactions between dystrophin glycoprotein complex proteins.
Academic Article Calmodulin-activated phosphorylation of dystrophin.
Academic Article The model calmodulin-binding peptide melittin inhibits phosphorylase kinase by interacting with its catalytic center.
Academic Article Ca2+-calmodulin binding to mouse alpha1 syntrophin: syntrophin is also a Ca2+-binding protein.
Academic Article Transcription factor-green fluorescent protein chimeric fusion proteins and their use in studies of DNA affinity chromatography.
Academic Article Apocalmodulin.
Academic Article Heparin elution of transcription factors from DNA-Sepharose columns.
Academic Article Phosphorylation of dystrophin and alpha-syntrophin by Ca(2+)-calmodulin dependent protein kinase II.
Academic Article DNA affinity chromatography.
Academic Article Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy.
Academic Article Calmodulin specifically binds three proteins of the dystrophin-glycoprotein complex.
Academic Article Oligomerization of mouse alpha 1-syntrophin and self-association of its pleckstrin homology domain 1 containing sequences.
Academic Article Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling.
Academic Article Plant calmodulin and the regulation of NAD kinase.
Academic Article Oligonucleotide trapping method for purification of transcription factors.
Academic Article Skeletal muscle signaling pathway through the dystrophin glycoprotein complex and Rac1.
Academic Article Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells.
Academic Article Effect of the detergent Tween-20 on the DNA affinity chromatography of Gal4, C/EBPalpha, and lac repressor with observations on column regeneration.
Academic Article Binding of laminin alpha1-chain LG4-5 domain to alpha-dystroglycan causes tyrosine phosphorylation of syntrophin to initiate Rac1 signaling.
Academic Article Alternate binding of actin and calmodulin to multiple sites on dystrophin.
Academic Article Laminin-alpha1 globular domains 3 and 4 induce heterotrimeric G protein binding to alpha-syntrophin's PDZ domain and alter intracellular Ca2+ in muscle.
Academic Article Ca2+-calmodulin binds to the carboxyl-terminal domain of dystrophin.
Academic Article Laminin-induced activation of Rac1 and JNKp46 is initiated by Src family kinases and mimics the effects of skeletal muscle contraction.
Academic Article Trapping of transcription factors with symmetrical DNA using thiol-disulfide exchange chemistry.
Academic Article Dystrophin glycoprotein complex-associated Gbetagamma subunits activate phosphatidylinositol-3-kinase/Akt signaling in skeletal muscle in a laminin-dependent manner.
Academic Article Two-dimensional southwestern blotting and characterization of transcription factors on-blot.
Academic Article Purification and identification of positive regulators binding to a novel element in the c-Jun promoter.
Concept Calcium-Binding Proteins
Concept Calmodulin-Binding Proteins
Concept Carrier Proteins
Concept DNA-Binding Proteins
Concept Protein Binding
Concept RNA-Binding Proteins
Concept GTP-Binding Proteins
Concept rho GTP-Binding Proteins
Concept rhoA GTP-Binding Protein
Concept cdc42 GTP-Binding Protein
Concept rac1 GTP-Binding Protein
Concept Heterotrimeric GTP-Binding Proteins
Concept CCAAT-Enhancer-Binding Proteins
Concept CCAAT-Enhancer-Binding Protein-alpha
Concept GTP-Binding Protein beta Subunits
Concept GTP-Binding Protein gamma Subunits
Academic Article Purification of the Ca2+-stimulated ATPase activator from human erythrocytes. Its membership in the class of Ca2+-binding modulator proteins.
Academic Article Human erythrocyte calmodulin. Further chemical characterization and the site of its interaction with the membrane.
Academic Article The synthesis and reaction of a specific affinity label for the hydrophobic drug-binding domains of calmodulin.
Academic Article Plant and fungal calmodulin: Ca2+-dependent regulation of plant NAD kinase.
Academic Article Evidence that calmodulin is in the chloroplast of peas and serves a regulatory role in photosynthesis.
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